PROJECT

Design and development of novel therapeutics for multidrug resistant bacteria

New therapeutics to treat multidrug resistant bacteria

Multi-drug resistance (MDR) in Gram-negative bacteria has been identified as a major worldwide public health concern.

Multi-drug resistant bacteria are responsible for approximately 700,000 deaths per year, a figure which could reach 10 million by the year 2050.

This project focuses on developing inhibitors of an endotoxin modifying enzyme that mediates antibiotic resistance by masking bacteria against both the human immune system and important classes of antibiotics. A structure guided approach will be used to develop novel therapeutic agents to treat MDR bacterial infections.

Through this project you will acquire skills in recombinant protein expression and purification. You will learn all aspects of protein crystallography including crystallisation, X-ray diffraction data collection and structure determination.

You will also obtain expertise in other biochemical and biophysical methods used to characterise the protein and its interactions with small molecule inhibitors.

As a multidisciplinary project, it provides opportunities for skills in structural biology, protein chemistry, biophysics and computational chemistry. You will work as a park of a team with medicinal chemists and microbiologists.

Find out more about research team leader, Professor Alice Vrielink or for more background information, see the suggested readings below.

Project goals

Elucidate structures of the enzyme in complex with small molecule inhibitors

Determine the binding affinities of the inhibitors for the enzyme

Use the structural information to optimise the inhibitors for enhanced potency

Research team leader: Professor Alice Vrielink

Our lab looks at the three-dimensional structures of proteins implicated in disease. We are focused on proteins that act as virulence factors causing antibiotic resistance as well as proteins that are important in causing cancer and tumorigenesis. We characterise the structures of these proteins and study their interactions with small molecule inhibitors as part of the process of structure-guided drug design.

 

News

PhD opportunities

Interested in becoming part of this project? Complete the following steps to submit your expression of interest:

Step 1 - Check criteria

General UWA PhD entrance requirements can be found on the Future Students website.

Requirements specific to this project:

  • a knowledge of proteins including their basic structures and function
  • biochemistry laboratory skills

Step 2 - Submit enquiry to research team leader

Step 3 - Lodge application

After you have discussed your project with the research team leader, you should be in a position to proceed to the next step of the UWA application process: Lodge an applicationDifferent application procedures apply to domestic and international students.

Scholarships

Collaboration and volunteers

Collaboration partners

Interested in volunteering?

Volunteers are welcome to help with protein production and crystallisation. Contact Professor Vrielink to register your interest.

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CRICOS Code: 00126G
Updated
Tuesday, 23 October 2018 1:35 AM (this date excludes nested assets)
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